TOPOII p48 (DM#261)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15026||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeBacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1384
Entrez GenePtf1a (a.k.a. PTF1-p, PTF1-p48, PTF1p48, bHLHa, bHLHa29)
- Cloning method TOPO Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
To make antisense probe: Cut with NotI and transcribe with Sp6 polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TOPOII p48 (DM#261) was a gift from Douglas Melton (Addgene plasmid # 15026 ; http://n2t.net/addgene:15026 ; RRID:Addgene_15026)
Map uploaded by the depositor.