pCS3 xenopus Mixer (DM#180?)
(Plasmid #15038)

• Depositing Lab: Douglas Melton
• Publication: Henry et al Science. 1998 Jul 3. 281(5373):91-6.

Backbone

• Vector backbone: pCS300 (modified pCS2)
• Backbone size w/o insert (bp): 4100
• Vector type: xenopus/mammalian/avian/zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mixer
• Alt name: Mix.3
• Species: X. laevis (frog)
• Insert Size (bp): 1450
• Entrez Gene: mixer (a.k.a. tMixer)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • Mixer In Situ Probe

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCS300 differs from pCS2 in that the polylinker II Not I site was moved to the polylinker I area and an Asc I and Pme I site were added to the polylinker II area.

You can use the CMV promoter in tissue culture cells or Xenopus embryos.

See the author's map for more cloning information. See the pdf attachment for information about making antisense probes.