Addgene - V44 pHIPPY blunt Plasmid Data

Plasmid 15098: V44 pHIPPY blunt

Gene/insert name:
None
Vector backbone:
pHIPPY
(Search Vector Database)
Backbone manufacturer:
Moon Lab
Vector type:
Mammalian Expression, RNAi
Backbone size (bp):
2167
5' sequencing primer:
H1 primer List of Sequencing Primers
Bacterial resistance(s)
Bleocin (Zeocin)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.

See article and author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. Kaykas et al (BMC Cell Biol. 2004 Apr 30. 5():16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15098" in your Materials and Methods section.