V44 pHIPPY blunt
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15098||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMoon Lab
- Backbone size (bp) 2167
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer H1 primer (Common Sequencing Primers)
Terms and Licenses
This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V44 pHIPPY blunt was a gift from Randall Moon (Addgene plasmid # 15098 ; http://n2t.net/addgene:15098 ; RRID:Addgene_15098)
For your References section:A plasmid-based system for expressing small interfering RNA libraries in mammalian cells. Kaykas A, Moon RT. BMC Cell Biol. 2004 Apr 30. 5():16. 10.1186/1471-2121-5-16 PubMed 15119963