Addgene - pLIA CRX (CC#69) Plasmid Data

Plasmid 15128: pLIA CRX (CC#69)

Gene/insert name:
CRX
Alt name:
cone-rod homeobox containing gene
Insert size:
1600
Species:
M. musculus (mouse)
Entrez Gene:
Crx (Crx1)
Vector backbone:
pLIA
(Search Vector Database)
Backbone manufacturer:
Cepko Lab
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6500
Cloning site 5':
EcoRV/SnaBI
Site destroyed during cloning:
Yes
Cloning site 3':
HincII/SnaBI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Comments:

An EcoRV-HincII Crx cDNA fragment containing the entire Crx coding region was subcloned into the SnaBI site of the pLIA retrovirus vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. Furukawa et al (Cell. 1997 Nov 14. 91(4):531-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15128" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

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