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pLIA EnR (CC#29)
(Plasmid #15152)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15152 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLIA
  • Backbone manufacturer
    Cepko Lab
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engrailed repressor
  • Alt name
    Engrailed
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1000
  • Mutation
    engrailed repressor domain
  • Entrez Gene
    en (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI/SnaBI (destroyed during cloning)
  • 3′ cloning site ClaI/SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIA EnR (CC#29) was a gift from Connie Cepko (Addgene plasmid # 15152 ; http://n2t.net/addgene:15152 ; RRID:Addgene_15152)
  • For your References section:

    Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation. Furukawa T, Morrow EM, Cepko CL. Cell. 1997 Nov 14. 91(4):531-41. 10.1016/S0092-8674(00)80439-0 PubMed 9390562