Addgene - Flag-Paxillin Plasmid Data

Plasmid 15212: Flag-Paxillin

Gene/insert name:
Paxillin
Insert size:
1700
Species:
G. gallus (chicken)
GenBank ID:
L30099
Entrez Gene:
PXN ()
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
Xho1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Chris Turner SUNY Upstate Medical
Sequence: View sequences (1)
Principal Investigator:
Rick Horwitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Paxillin phosphorylation sites mapped by mass spectrometry. Webb et al (J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15212" in your Materials and Methods section.