pGAL4-CREB S133A
(Plasmid #15222)

• Depositing Lab: Ugo Moens
• Publication: Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Backbone

• Vector backbone: pM
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CREB S133A
• Alt name: CREB
• Species: R. norvegicus (rat)
• Insert Size (bp): 1000
• Mutation: Serine 133 mutated to Alanine
• Entrez Gene: Creb1 (a.k.a. Creb)
• Tag / Fusion Protein:
  • Gal4 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI/SalI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Gal4-N-term primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CREB kindly provided by Richard A. Maurer
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wild-type CREB S133A fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the CREB S133A as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.