Addgene - pGAL4-CREB S133A Plasmid Data

Plasmid 15222: pGAL4-CREB S133A

Gene/insert name:
CREB S133A
Alt name:
CREB
Insert size:
1000
Species:
R. norvegicus (rat)
Entrez Gene:
Creb1 (Creb)
Fusion protein or tag:
Gal4
Terminal:
N terminal on backbone
Mutation:
Serine 133 mutated to Alanine
Vector backbone:
pM
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3500
Cloning site 5':
XhoI/SalI
Site destroyed during cloning:
Yes
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
Gal4-N-term primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
CREB kindly provided by Richard A. Maurer
Sequence: View sequences (1)
Principal Investigator:
Ugo Moens
Terms and Licenses:
MTA

Comments:

Wild-type CREB S133A fused to the DNA binding domain of Gal4. The plasmids were generated by cloning the CREB S133A as an XhoI/XbaI fragment into the SalI/XbaI sites of pM.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The cAMP signalling pathway activates CREB through PKA, p38 and MSK1 in NIH 3T3 cells. Delghandi et al (Cell Signal. 2005 Nov . 17(11):1343-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15222" in your Materials and Methods section.