Addgene - Flag-ECFP-GIT1 Plasmid Data

Plasmid 15223: Flag-ECFP-GIT1

Gene/insert name:
G protein coupled receptor kinase-interacting protein 1
Alt name:
GIT1
Insert size:
2200
Species:
H. sapiens (human)
Entrez Gene:
GIT1 ()
Fusion protein or tag:
flag
Terminal:
N terminal on backbone
Fusion protein or tag:
ECFP
Terminal:
N terminal on backbone
Vector backbone:
pCDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6100
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
Not1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Rick Horwitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of phosphorylation sites in GIT1. Webb et al (J Cell Sci. 2006 Jul 15. 119(Pt 14):2847-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15223" in your Materials and Methods section.