Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15234||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namePAK interactive exchange factor
SpeciesM. musculus (mouse)
Insert Size (bp)1950
Entrez GeneArhgef7 (a.k.a. Cool, PIX, Pak3bp, betaPix, betaPix-b, betaPix-c, cool-1, mKIAA0142, p85Cool1, p85SPR)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag-betaPIXa was a gift from Rick Horwitz (Addgene plasmid # 15234 ; http://n2t.net/addgene:15234 ; RRID:Addgene_15234)
For your References section:Identification of protein networks associated with the PAK1-betaPIX-GIT1-paxillin signaling complex by mass spectrometry. Mayhew MW, Webb DJ, Kovalenko M, Whitmore L, Fox JW, Horwitz AF. J Proteome Res. 2006 Sep . 5(9):2417-23. 10.1021/pr060140t PubMed 16944954