Addgene - Flag-ECFP-PAK1 Plasmid Data

Plasmid 15237: Flag-ECFP-PAK1

Gene/insert name:
p21-activated kinase
Insert size:
800
Species:
H. sapiens (human)
Entrez Gene:
PAK1 (PAKalpha)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
ECFP
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6100
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
Xho1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. Jonathan Chernoff, Fox Chase Cancer Center, Philadelphia, PA
Sequence: View sequences (2)
Principal Investigator:
Rick Horwitz
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15237" in your Materials and Methods section.