Addgene - pMKO.1-Puro-shPP2A-Aalpha Plasmid Data

Plasmid 15249: pMKO.1-Puro-shPP2A-Aalpha

Gene/insert name:
PP2A regulatory subunit A, alpha isoform
Alt name:
PP2A A alpha
Insert size:
58
Species:
H. sapiens (human)
Entrez Gene:
PPP2R1A (PP2A-Aalpha, PP2AAALPHA, PR65A)
Vector backbone:
pMKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6700
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Comments:

Target sequence: GACAACAGCACCTTGCAGAGT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The tumor suppressor PP2A Abeta regulates the RalA GTPase. Sablina et al (Cell. 2007 Jun 1. 129(5):969-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15249" in your Materials and Methods section.