Addgene - pBabe-Puro-BRAF-V600E Plasmid Data

Plasmid 15269: pBabe-Puro-BRAF-V600E

Gene/insert name:
BRAF
Insert size:
2400
Species:
H. sapiens (human)
Entrez Gene:
BRAF (B-RAF1, BRAF1, NS7, RAFB1)
Fusion protein or tag:
Myc
Terminal:
C terminal on insert
Fusion protein or tag:
His
Terminal:
C terminal on insert
Mutation:
Valine 600 to Glutamate
Vector backbone:
pBabe-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
HindIII
Site destroyed during cloning:
Yes
Cloning site 3':
HindIII
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm et al (Cell. 2007 Jun 15. 129(6):1065-1079. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15269" in your Materials and Methods section.