Addgene - pCaST-elav-Gal4AD Plasmid Data

Plasmid 15307: pCaST-elav-Gal4AD

Gene/insert name:
Gal4AD-ZIP+
Alt name:
GAL4AD-EE12RR345L
Alt name:
GAL4
Insert size:
670
Entrez Gene:
GAL4 (YPL248C, GAL81)
Vector backbone:
pCaST-elav
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
13475
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
AscI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Benjamin White
Terms and Licenses:
MTA

Comments:

The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15307" in your Materials and Methods section.