AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
(Plasmid #154001)

• Purpose: Vector expressed CasRx and sgRNA_Ptbp1 for AAV package
• Depositing Lab: Hui Yang
• Publication: Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.

Backbone

• Vector backbone: pAAV
• Vector type: Mouse Targeting, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CasRx, Ptbp1 sgRNAs
• Alt name: NLS-RfxCas13d-NLS
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 3849
• Promoter: GFAP, U6
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • 2 EP371 AAV-GFAP-CasRx-Ptbp1.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

INSERT 1: CasRx driven by GFAP promoter.
INSERT 2: DR-SgRNA_Ptbp1 driven by U6 promoter.
Ptbp1 gRNA 5: 5′-tgtagatgggctgtccacgaagcactggcg-3′
Ptbp1 gRNA 6: 5′-gcttggagaagtcgatgcgcagcgtgcagc-3′