pJH1
(Plasmid
#154204)
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Purpose(Empty Backbone) encodes CRISPR-Cas9 system derived from plasmid pCas9
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCas9
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Modifications to backboneAdded an origin of replication from pUCP22; chloramphenicol resistance gene removed and replaced with kanamycin resistance; a portion of the Cas9 protein-encoding region was resynthesized, conserving the encoded amino acid sequence but altering the GC content to 48% (from ~35%); 103bp deleted between the tracrRNA and the Cas9 encoding region.
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJH1 was a gift from Russell Poulter (Addgene plasmid # 154204 ; http://n2t.net/addgene:154204 ; RRID:Addgene_154204) -
For your References section:
The application of the CRISPR-Cas9 system in Pseudomonas syringae pv. actinidiae. Ho J, Zhao M, Wojcik S, Taiaroa G, Butler M, Poulter R. J Med Microbiol. 2020 Mar;69(3):478-486. doi: 10.1099/jmm.0.001124. Epub 2020 Jan 14. 10.1099/jmm.0.001124 PubMed 31935181
Map uploaded by the depositor.
