Addgene - -227 hTF (mutant NF-kB) Plasmid Data

Plasmid 15444: -227 hTF (mutant NF-kB)

Gene/insert name:
Tissue factor promoter
Alt name:
TF
Insert size:
348
Species:
H. sapiens (human)
Entrez Gene:
F3 (CD142, TF, TFA)
Mutation:
NF-kappaB binding domain mutated
Vector backbone:
pGL2 Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
5600
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Nigel Mackman
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

-227 to +121 hTF promoter with NF-kappaB binding domain mutated. hTF promoter deletion series, Mackman lab #2587.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of the tissue factor gene in human monocytic cells. Role of AP-1, NF-kappa B/Rel, and Sp1 proteins in uninduced and lipopolysaccharide-induced expression. Oeth et al (Arterioscler Thromb Vasc Biol. 1997 Feb . 17(2):365-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15444" in your Materials and Methods section.