pCDNA5/tom20-pbira(79-c)-ha
(Plasmid #154456)

• Purpose: expresses tom20-pbira(79-c)-ha/biochemical assay
• Depositing Lab: Hyun-Woo Rhee
• Publication: Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. pii: 1916584117. doi: 10.1073/pnas.1916584117.

Backbone

• Vector backbone: pCDNA5
• Backbone size w/o insert (bp): 5082
• Total vector size (bp): 6312
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tom20-pbira(79-c)-ha
• Species: H. sapiens (human)
• Insert Size (bp): 1230
• GenBank ID: NM_014765.2
• Entrez Gene: TOMM20 (a.k.a. MAS20, MOM19, TOM20)
• Promoter: CMV-F

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII, KpnI (not destroyed)
• 3′ cloning site: NotI, XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter, CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry