Addgene - pCL25 Plasmid Data

Plasmid 15456: pCL25

Gene/insert name:
None
GenBank ID:
EF540894
Vector backbone:
N/A
(Search Vector Database)
Vector type:
S. aureus-E. coli shuttle vector
Backbone size (bp):
6624
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
tetracycline/ S. aureus
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Chia Lee
Terms and Licenses:
MTA

Comments:

pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Improved single-copy integration vectors for Staphylococcus aureus. Luong et al (J Microbiol Methods. 2007 Jul . 70(1):186-90. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15456" in your Materials and Methods section.