Addgene - Dopamine Receptor D1 Flag Plasmid Data

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Plasmid 15464: Dopamine Receptor D1 Flag

Gene/insert name:
Dopamine Receptor D1
Insert size:
1340
Species:
H. sapiens (human)
GenBank ID:
NM_000794
Entrez Gene:
DRD1 (DADR, DRD1A)
Fusion protein or tag:
FLAG
Terminal:
C terminal on backbone
Vector backbone:
pcDNA5/FRT/TO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5137
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Wei-dong Yao
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inhibition of the dopamine D1 receptor signaling by PSD-95. Zhang et al (J Biol Chem. 2007 May 25. 282(21):15778-89. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15464" in your Materials and Methods section.