Dopamine Receptor D1 Flag
(Plasmid #15464)

• Depositing Lab: Wei-dong Yao
• Publication: Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-89.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dopamine Receptor D1
• Species: H. sapiens (human)
• Insert Size (bp): 1340
• GenBank ID: NM_000794
• Entrez Gene: DRD1 (a.k.a. D1R, DADR, DRD1A)
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry