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pCS2-hDKK3-flag
(Plasmid #15496)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15496 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DKK3
  • Alt name
    Dickkopf-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1130
  • Mutation
    See note below
  • GenBank ID
    AF177396
  • Entrez Gene
    DKK3 (a.k.a. REIC, RIG)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

See author's map for details.

Addgene NGS identified G347R variant compared to NCBI reference [AAF02676.1].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hDKK3-flag was a gift from Sergei Sokol (Addgene plasmid # 15496 ; http://n2t.net/addgene:15496 ; RRID:Addgene_15496)
  • For your References section:

    Functional and structural diversity of the human Dickkopf gene family. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Gene. 1999 Oct 1. 238(2):301-13. 10.1016/S0378-1119(99)00365-0 PubMed 10570958