pSR prdm3 (PRDM16 shRNA)
(Plasmid #15505)

• Depositing Lab: Bruce Spiegelman
• Publication: Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Backbone

• Vector backbone: pSuper-Retro Neo + GFP
• Backbone manufacturer: oligoengine
• Backbone size w/o insert (bp): 7300
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Neomycin (select with G418) ; GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRDM16 shRNA
• Alt name: PRDM16
• Alt name: Mel1
• Species: M. musculus (mouse)
• Insert Size (bp): 50
• Entrez Gene: Prdm16 (a.k.a. 5730557K01Rik, csp1, mel1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: MSCV rev
• 3′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The siRNA sequence for use in PRDM16 depletion experiments was 5'-GAAGAGCGUGAGUACAAAU-3'. The corresponding double-stranded DNA sequence was ligated into pSUPER-Retro (GFP-Neo) (OligoEngine) for retroviral shRNA expression. This siRNA sequence was able to specifically knock down PRDM16 protein and mRNA expression by over 75%.