Addgene - pSR prdm3 (PRDM16 shRNA) Plasmid Data

Plasmid 15505: pSR prdm3 (PRDM16 shRNA)

Gene/insert name:
PRDM16 shRNA
Alt name:
PRDM16
Alt name:
Mel1
Insert size:
50
Species:
M. musculus (mouse)
Entrez Gene:
Prdm16 (RP23-351I23.3, 5730557K01Rik, C130091E20, csp1, mKIAA1675, mel1)
Vector backbone:
pSuper-Retro Neo + GFP
(Search Vector Database)
Backbone manufacturer:
oligoengine
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
7300
Cloning site 5':
BglII
Site destroyed during cloning:
Yes
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
MSCV rev List of Sequencing Primers
3' sequencing primer:
pBABE 5'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Selectable markers, other:
GFP
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA

Comments:

The siRNA sequence for use in PRDM16 depletion experiments was 5'-GAAGAGCGUGAGUACAAAU-3'. The corresponding double-stranded DNA sequence was ligated into pSUPER-Retro (GFP-Neo) (OligoEngine) for retroviral shRNA expression. This siRNA sequence was able to specifically knock down PRDM16 protein and mRNA expression by over 75%.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transcriptional Control of Brown Fat Determination by PRDM16. Seale et al (Cell Metab. 2007 Jul . 6(1):38-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15505" in your Materials and Methods section.