Plasmid 15569: GPD 104QDProGFP p416

• Gene/insert name: htt 104Q Delta Pro
• Alt name: htt 104Q
• Species: H. sapiens (human)
• GenBank ID: NM_002111
• Entrez Gene: HTT (HD, IT15)
  
• Fusion protein or tag: GFP
• Terminal: C terminal on insert
• Mutation: Proline-rich region removed
• Vector backbone: p416 GPD
  (Search Vector Database)
• Vector type: Yeast Expression
• Backbone size w/o insert (bp): 5800
• 5' sequencing primer: GPDpro-F
• 3' sequencing primer: EGFP-N
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: Low Copy
• Selectable markers: URA3
• Sequence: View sequences (1)
• Principal Investigator: Susan Lindquist
• Terms and Licenses: MTA

Comments: 

Figure 2, III in article.

Lindquist lab number: 7537

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.

Article: Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald et al (Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. PubMed)