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GAL FLAG103Q+ProGFPp303
(Plasmid #15573)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    htt 103Q + Pro
  • Alt name
    htt 103Q
  • Species
    H. sapiens (human)
  • Mutation
    Proline-rich region present
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Figure 2, IV in article.

Lindquist lab number: 7698

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAL FLAG103Q+ProGFPp303 was a gift from Susan Lindquist (Addgene plasmid # 15573)
  • For your References section:

    Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. 10.1073/pnas.0604547103 PubMed 16832050