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Plasmid 15573: GAL FLAG103Q+ProGFPp303
  • htt 103Q + Pro

  • htt 103Q

  • H. sapiens (human)

  • NM_002111

  • HTT (HD, IT15)

  • FLAG

  • N terminal on insert

  • GFP

  • C terminal on insert

  • Proline-rich region present

  • p303 Gal
    (Search Vector Database)

  • Yeast Expression

  • Gal1 List of Sequencing Primers

  • EGFP-N

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • HIS3

  • View sequences (1)
  • Susan Lindquist

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Figure 2, IV in article.

Lindquist lab number: 7698

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald et al (Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15573" in your Materials and Methods section.