Addgene - GAL FLAG103Q+ProGFPp303 Plasmid Data

Plasmid 15573: GAL FLAG103Q+ProGFPp303

Gene/insert name:
htt 103Q + Pro
Alt name:
htt 103Q
Species:
H. sapiens (human)
GenBank ID:
NM_002111
Entrez Gene:
HTT (HD, IT15)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
Proline-rich region present
Vector backbone:
p303 Gal
(Search Vector Database)
Vector type:
Yeast Expression
5' sequencing primer:
Gal1 List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
HIS3
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

Figure 2, IV in article.

Lindquist lab number: 7698

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald et al (Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15573" in your Materials and Methods section.