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Plasmid 15585: GAL FLAG46QDProCFPp303
  • htt 46Q Delta Pro

  • htt 46Q

  • H. sapiens (human)

  • NM_002111

  • HTT (HD, IT15)

  • FLAG

  • N terminal on insert

  • CFP

  • C terminal on insert

  • Proline-rich region removed

  • p303 Gal
    (Search Vector Database)

  • Yeast Expression

  • Gal1 List of Sequencing Primers

  • EGFP-N

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • HIS3

  • View sequences (1)
  • Susan Lindquist

  • MTA

Comments: 

Figure 1C in article.

Lindquist lab number: 7731

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald et al (Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15585" in your Materials and Methods section.