pInducer20_SLC38A2_N82A
(Plasmid #156183)

• Purpose: Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.
• Depositing Lab: Alec Kimmelman
• Publication: Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 10.1158/2159-8290.CD-19-0959.

Backbone

• Vector backbone: pInducer20
• Backbone size w/o insert (bp): 11000
• Total vector size (bp): 11669
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC38A2
• Alt name: SNAT2
• Species: H. sapiens (human)
• Insert Size (bp): 1521
• Mutation: Silent mutations to prevent targeting by sgRNA: TAATCTGAGCAATGCGATTG. N82A (AAT --> GCT) "transport defective" mutation.
• Entrez Gene: SLC38A2 (a.k.a. ATA2, PRO1068, SAT2, SNAT2)
• Promoter: TRE

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX

Resource Information

• Supplemental Documents:
  • pInducer20_resSLC38A2_N82A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry