pFLAG-CMV2 ACAP1
(Plasmid #15697)

• Depositing Lab: Paul Randazzo
• Publication: Jackson et al J Cell Biol. 2000 Oct 30. 151(3):627-38.

Backbone

• Vector backbone: pFlag-CMV2
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4679
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACAP1
• Alt name: centaurin, beta 1
• Species: H. sapiens (human)
• Entrez Gene: ACAP1 (a.k.a. CENTB1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ACAP1/KIAA0050 cDNA was obtained from Dr. Takahiro Nagese at the Kazusa DNA Research Institute (Chiba, Japan)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry