Addgene - pcDNA3-DN-hCUL3-FLAG Plasmid Data

Plasmid 15820: pcDNA3-DN-hCUL3-FLAG

Gene/insert name:
Cullin3
Alt name:
Cullin 3
Alt name:
Cul3
Insert size:
1250
Species:
H. sapiens (human)
Entrez Gene:
CUL3 (CUL-3, PHA2E)
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Mutation:
Dominant negative: N418
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
BglII/BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA

Comments:

C-terminal truncation of hCul3: amino acids 1-418.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of substrates for F-box proteins. Jin et al (Methods Enzymol. 2005 . 399():287-309. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15820" in your Materials and Methods section.