pGEX-S1*HiGlpG
(Plasmid #158535)

• Purpose: Bacterial expression of rhomboid protease mutant S1*HiGlpG
• Depositing Lab: Sinisa Urban
• Publication: Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(20)30279-3. doi: 10.1016/j.str.2020.07.015.

Backbone

• Vector backbone: PGEX-6P-1
• Backbone size w/o insert (bp): 4953
• Total vector size (bp): 5532
• Modifications to backbone: -
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S1*HiGlpG
• Species: Synthetic
• Insert Size (bp): 579
• Mutation: Sector 1 mutations
• Tag / Fusion Protein:
  • GST tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: - (destroyed during cloning)
• 3′ cloning site: - (destroyed during cloning)
• 5′ sequencing primer: PGEX-5'
• 3′ sequencing primer: PGEX-3'

Resource Information

• Supplemental Documents:
  • pgex-s1higlpg.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry