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pCR-Zeo
(Plasmid #158709)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 158709 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSL001
  • Backbone manufacturer
    from Houhui Song's Lab
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology ; Mycobacteria Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crRNA
  • gRNA/shRNA sequence
    gtctaagaactttaaataatttctactgttgtagattccgtctccggcctccagtagtattcggcgaagcttgtctaagaactttaaataatttctactgttgtagat
  • Species
    Other
  • Promoter Hsp60

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that several differences were identified in the plasmid backbone compared to the depositor's sequence. These differences do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-Zeo was a gift from Yi-Cheng Sun (Addgene plasmid # 158709 ; http://n2t.net/addgene:158709 ; RRID:Addgene_158709)
  • For your References section:

    CRISPR-Cas12a-Assisted Recombineering in Bacteria. Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC. Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 10.1128/AEM.00947-17. Print 2017 Sep 1. 10.1128/AEM.00947-17 PubMed 28646112