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pCDFDuet-1::ADAT3-ADAT2
(Plasmid #15917)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15917 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3781
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADAT3 & ADAT2
  • Species
    T. brucei
  • Insert Size (bp)
    1097
  • Tags / Fusion Proteins
    • HexaHistidine (N terminal on backbone)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI & NdeI (not destroyed)
  • 3′ cloning site NotI & XhoI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDFDuet-1::ADAT3-ADAT2 was a gift from Nina Papavasiliou (Addgene plasmid # 15917)
  • For your References section:

    An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Rubio MA, Pastar I, Gaston KW, Ragone FL, Janzen CJ, Cross GA, Papavasiliou FN, Alfonzo JD. Proc Natl Acad Sci U S A. 2007 May 8. 104(19):7821-6. 10.1073/pnas.0702394104 PubMed 17483465