psiCHECK2-2A-3xFLAG-XBP1u_pause-2A
(Plasmid #159583)

• Purpose: Luciferase reporter for ribosome pausing
• Depositing Lab: Shintaro Iwasaki
• Publication: Han et al Cell Rep. 2020 May 5;31(5):107610. doi: 10.1016/j.celrep.2020.107610.

Backbone

• Vector backbone: psiCHECK2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 6279
• Total vector size (bp): 5698
• Modifications to backbone: Depletion of synthetic poly(A) signal and HSV-TK promoter
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: XBP1u pause site
• Species: H. sapiens (human), M. musculus (mouse)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATGACGATGACAAGGCCTGGAGGAGCTCCC
• 3′ sequencing primer: AGTAGCTCCGCTTCCGGTCAAAACGAATGAGTTCATTAATGGCTTCCAGCTT

Gene/Insert 2

• Gene/Insert name: EIF2S3 5UTR
• Species: H. sapiens (human)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATACGACTCACTATAGGTTTCCTTCCTCTTTTGGCAACATGGCTTCCAAGGTGTA
• 3′ sequencing primer: TACACCTTGGAAGCCATGTTGCCAAAAGAGGAAGGAAACCTATAGTGAGTCGTAT

Resource Information

• Supplemental Documents:
  • psiCHECK2_1_2A_3xFLAG_XBP1u_2A.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry