pKP515
(Plasmid
#159597)
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PurposeExpress E.coli FepA protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)KP1394
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Growth instructionsKP1394 is W3110 fepA::kan, recA::cat. Plasmids can also be maintained in DH5alpha.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefepA
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SpeciesEscherichia coli
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Mutationnone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP515 was a gift from Kathleen Postle (Addgene plasmid # 159597 ; http://n2t.net/addgene:159597 ; RRID:Addgene_159597) -
For your References section:
FepA with globular domain deletions lacks activity. Vakharia HL, Postle K. J Bacteriol. 2002 Oct;184(19):5508-12. doi: 10.1128/jb.184.19.5508-5512.2002. 10.1128/jb.184.19.5508-5512.2002 PubMed 12218040