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p405MET3
(Plasmid #15962)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15962 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p405MET3b
  • Backbone manufacturer
    A. Geoghegan (Rockefeller University)
  • Backbone size w/o insert (bp) 5578
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET3 promoter + polylinker + CYC1 terminator
  • Alt name
    ATP sulfurylase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    649
  • Mutation
    MET3 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    MET3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NgoMIV (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original vector p405MET3b was created by subcloning PCR fragment of genomic MET3 promoter (with SacI and XbaI restriction sites introduced) into SacI-XbaI cut vector pRS405. This plasmid does not contains CYC1 terminator. Plasmid p405MET3 was created by subcloning XbaI-NgoMIV insert from p405GALS (insert = polylinker + CYC1 terminator) into XbaI-NgoMIV cut p405MET3b vector.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p405MET3 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15962)