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Purpose(Empty Backbone) CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJOE8999
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Vector typeCRISPR ; shuttle vector
- Promoter Constitutive tracrRNA
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsBacillus subtilis
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Copy numberLow Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRH030 was a gift from Robert Hertel (Addgene plasmid # 159633 ; http://n2t.net/addgene:159633 ; RRID:Addgene_159633) -
For your References section:
A CRISPR-Cas9 tool to explore the genetics of Bacillus subtilis phages. Otte K, Kuhne NM, Furrer AD, Baena Lozada LP, Lutz VT, Schilling T, Hertel R. Lett Appl Microbiol. 2020 Jul 2. doi: 10.1111/lam.13349. 10.1111/lam.13349 PubMed 32615024
