|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15972||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerSikorski & Hieter, Genetics 122:19-27 (1989)
- Backbone size w/o insert (bp) 4173
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTEF1 promoter + polylinker + CYC1 terminator
Alt nameTranslational elongation factor EF-1 alpha
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)728
MutationTEF1 promoter is between SacI and XbaI restriction sites.
Entrez GeneTEF1 (a.k.a. YPR080W)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPlasmid was made by Ben Timney (Rockefeller University). Insert was cut from Mumberg plasmid p413TEF, which was obtained from ATCC.
Terms and Licenses
Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p404TEF1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15972)