Addgene - pCMV6 Myc PAK5 K478M Plasmid Data

Plasmid 16020: pCMV6 Myc PAK5 K478M

Gene/insert name:
PAK5
Insert size:
2200
Species:
H. sapiens (human)
Entrez Gene:
PAK7 (RP5-1119D9.3, PAK5)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Mutation:
K478M: kinase dead
Vector backbone:
pCMV6-myc
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4900
Cloning site 5':
BamHI/BglII
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jonathan Chernoff
Terms and Licenses:
MTA

Comments:

Order of elements: SalI-Myc-XbaI-Pak5-EcoRI

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD. Cotteret et al (Mol Cell Biol. 2003 Aug . 23(16):5526-39. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16020" in your Materials and Methods section.