pLEX307-SARS-CoV-2-3CL-C145A
(Plasmid #160279)

• Purpose: Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus
• Depositing Lab: Alejandro Chavez
• Publication: Resnick et al bioRxiv. 2020 Aug 29. doi: 10.1101/2020.08.29.272864.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	160279	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pLEX_307
  (Search Vector Database)
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable ; NEB 10Beta
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3CL Protease
• Species: SARS-CoV-2
• Insert Size (bp): 924
• Mutation: C145A
• Promoter: EF1a

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pLEX307-SARS-CoV-2-3CL-C145A.gb
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry