p4RTK GL4.10 (Luc2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16057||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL4.10 (luc2)
- Backbone size w/o insert (bp) 4200
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMultimerized E-box
SpeciesM. musculus (mouse)
Insert Size (bp)250
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Contains three repeats of 5'-TTGGGAGGCAGCAGGTG-3'. p4RTK: has a minimal thymidine kinase promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4RTK GL4.10 (Luc2) was a gift from Robert Benezra (Addgene plasmid # 16057 ; http://n2t.net/addgene:16057 ; RRID:Addgene_16057)
For your References section:Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1. Tournay O, Benezra R. Mol Cell Biol. 1996 May . 16(5):2418-30. PubMed 8628310