pTRE-Tight caspase-3 (p12)::nz [TU#817]
(Plasmid #16084)

• Depositing Lab: Martin Chalfie
• Publication: Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8.

Backbone

• Vector backbone: pTRE-tight
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2540
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: caspase-3 (p12) :: nz
• Alt name: caspase-3
• Species: H. sapiens (human)
• Insert Size (bp): 477
• Entrez Gene: CASP3 (a.k.a. CPP32, CPP32B, SCA-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: LNCX

Resource Information

• A portion of this plasmid was derived from a plasmid made by: caspase-3 from MGC full length cDNA clone ID 4419175.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

caspase-3 (p12) fused to the antiparallel leucine-zipper domain NZ (ALKKELQANKKELAQLKWELQALKKELAQ). For listed sequence, 356-664bp = caspase3(p12); 728-820bp = NZ.