pBSc + MShab
(Plasmid #16146)

• Depositing Lab: Larry Salkoff
• Publication: Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Backbone

• Vector backbone: pBSc
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mus musculus potassium voltage gated channel
• Alt name: GI:200975
• Alt name: M64228
• Species: M. musculus (mouse), D. melanogaster (fly)
• Insert Size (bp): 3400
• Mutation: N/A
• GenBank ID: NM_008420
• Entrez Gene: Kcnb1 (a.k.a. Kcr1-1, Kv2.1, Shab)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sac11 (not destroyed)
• 3′ cloning site: Hind111 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry