pM2AH + mSlo1
(Plasmid #16152)

• Depositing Lab: Larry Salkoff
• Publication: Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Backbone

• Vector backbone: pM2-AH
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mus musculus potassium large conductance calcium-activated channel
• Alt name: mSlo1
• Alt name: GeneID:16531
• Alt name: MGI:99923
• Species: M. musculus (mouse)
• Insert Size (bp): 4740
• Mutation: N/A
• GenBank ID: NM_010610
• Entrez Gene: Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry