Addgene - pGEX2TK-PDZ(HtrA2) Plasmid Data

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16207: pGEX2TK-PDZ(…
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Plasmid 16207: pGEX2TK-PDZ(HtrA2)

Gene/insert name:
HtrA2
Alt name:
Omi
Insert size:
320
Species:
H. sapiens (human)
GenBank ID:
NM_013247
Entrez Gene:
HTRA2 (OMI, PARK13, PRSS25)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
amino acids 350–459
Vector backbone:
pGEX-2TK
(Search Vector Database)
Backbone manufacturer:
Pharmacia
Vector type:
Bacterial Expression
Backbone size w/o insert:
4950
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX 5' Sequencing primer List of Sequencing Primers
3' sequencing primer:
pGEX 3' Sequencing primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
L. Miguel Martins
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi. Martins et al (J Biol Chem. 2003 Dec 5. 278(49):49417-27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16207" in your Materials and Methods section.