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pJYDNgp
(Plasmid #162453)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162453 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    New backbone pJYD
  • Backbone manufacturer
    Dr. Jiri Zahradnik
  • Backbone size w/o insert (bp) 5230
  • Total vector size (bp) 5231
  • Modifications to backbone
    Deletion of two stop codons and the addition of one nucleotide in the MCS site.
  • Vector type
    Shuttle vector bacteria/yeast
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aga2p-2Glinker-eUnaG2
  • Insert Size (bp)
    915
  • Entrez Gene
    AGA2 (a.k.a. YGL032C)
  • Promoter GAL1
  • Tags / Fusion Proteins
    • HA nad myc (C terminal on insert)
    • eUnaG2 fluorescent protein (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJYDNgp was a gift from Gideon Schreiber (Addgene plasmid # 162453 ; http://n2t.net/addgene:162453 ; RRID:Addgene_162453)
  • For your References section:

    An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures. Zahradník J, Dey D, Marciano S, Schreiber G. bioRxiv Preprint 2020 10.1101/2020.12.16.423176