Addgene - pcDNA3 236HSC WT (BRCA2) Plasmid Data

Plasmid 16246: pcDNA3 236HSC WT (BRCA2)

Gene/insert name:
BRCA2
Insert size:
10000
Species:
H. sapiens (human)
Entrez Gene:
BRCA2 (RP11-298P3.4, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1, GLM3, PNCA2)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGHrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Comments:

To construct p236BRCA2, the pcDNA3 vector was first modified by inserting a 236-bp fragment of the 5' untranslated region of BRCA2 between the KpnI and NotI sites. The assembled full-length BRCA2 cDNA was then inserted at the XhoI site of this plasmid. The 5' UTR of BRCA2 was obtained by RT-PCR using primers 5'-GGTACCGGTG GCGCGAGCTT CTGA-3' and 5'-GCGGCCGCAA CTACGATATT CCTCCAAT-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inhibition of cancer cell growth by BRCA2. Wang et al (Cancer Res. 2002 Mar 1. 62(5):1311-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16246" in your Materials and Methods section.