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pNeuLite
(Plasmid #16247)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16247 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Her-2/neu promoter
  • Alt name
    Her2
  • Species
    H. sapiens (human)
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The promoter region of human HER-2/neu was amplified with a pair of primers (KO11: 5'−GATAGGATCC GGGGGTCCTG GAAGCC−3' and KO2: 5'−GGGCAGATCT GGTTTTCCGG TCCCAATGGA−3'). Also named pNeuLit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNeuLite was a gift from Mien-Chie Hung (Addgene plasmid # 16247 ; http://n2t.net/addgene:16247 ; RRID:Addgene_16247)
  • For your References section:

    The ets protein PEA3 suppresses HER-2/neu overexpression and inhibits tumorigenesis. Xing X, Wang SC, Xia W, Zou Y, Shao R, Kwong KY, Yu Z, Zhang S, Miller S, Huang L, Hung MC. Nat Med. 2000 Feb . 6(2):189-95. 10.1038/72294 PubMed 10655108