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pmiCas9
(Plasmid #162758)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162758 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJDS246
  • Backbone manufacturer
    Joung lab
  • Backbone size w/o insert (bp) 2466
  • Total vector size (bp) 7722
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miCas9
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5387
  • Mutation
    Contains Brex27 sequence range is 4958-5065
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original gene was from Addgene plasmid#47754

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiCas9 was a gift from Eugene Chen (Addgene plasmid # 162758 ; http://n2t.net/addgene:162758 ; RRID:Addgene_162758)
  • For your References section:

    MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Ma L, Ruan J, Song J, Wen L, Yang D, Zhao J, Xia X, Chen YE, Zhang J, Xu J. Nat Commun. 2020 Nov 27;11(1):6082. doi: 10.1038/s41467-020-19842-2. 10.1038/s41467-020-19842-2 PubMed 33247137