Addgene - pBS mouse ER81 [TJ#197] Plasmid Data

Plasmid 16282: pBS mouse ER81 [TJ#197]

Gene/insert name:
ER81
Insert size:
2500
Species:
M. musculus (mouse)
Entrez Gene:
Etv1 (ER81, Etsrp81)
Vector backbone:
pBluescript SK
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Thomas Jessell
Terms and Licenses:
MTA

Comments:

To make a sense probe, cut with XhoI and transcribe with T3. To make an antisense probe, cut with SpeI and transcribe with T7.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16282" in your Materials and Methods section.