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pCMV-HA GCNF
(Plasmid #16336)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16336 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-HA
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCNF
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nr6a1 (a.k.a. 1700113M01Rik, Gcnf, NCNF, RTR)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Compared to GenBank accession number NP_034394.1, the GCNF insert in this plasmid contains N33S and N414D mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA GCNF was a gift from Austin Cooney (Addgene plasmid # 16336)
  • For your References section:

    Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu P, LeMenuet D, Chung AC, Mancini M, Wheeler DA, Cooney AJ. Mol Cell Biol. 2005 Oct . 25(19):8507-19. 10.1128/MCB.25.19.8507-8519.2005 PubMed 16166633