Addgene - pHA-mLRH-1 Plasmid Data

Plasmid 16342: pHA-mLRH-1

Gene/insert name:
LRH-1
Species:
M. musculus (mouse)
Entrez Gene:
Nr5a2 (AU020803, D1Ertd308e, Ftf, LRH-1)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pCMV-HA
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3800
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Austin Cooney
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic development. Gu et al (Mol Cell Biol. 2005 May . 25(9):3492-505. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16342" in your Materials and Methods section.