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pGEM-Nanog
(Plasmid #16351)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16351 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens Nanog homeobox
  • Alt name
    NANOG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • GenBank ID
    NM_024865
  • Entrez Gene
    NANOG

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains the Nanog sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-Nanog was a gift from James Thomson (Addgene plasmid # 16351 ; http://n2t.net/addgene:16351 ; RRID:Addgene_16351)
  • For your References section:

    Induced pluripotent stem cell lines derived from human somatic cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA. Science. 2007 Dec 21. 318(5858):1917-20. 10.1126/science.1151526 PubMed 18029452